Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.
Identifieur interne : 000136 ( Main/Exploration ); précédent : 000135; suivant : 000137Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.
Auteurs : Yujie Li [République populaire de Chine] ; Hui Ren [République populaire de Chine] ; Yutao Zhao [République populaire de Chine] ; Jiyuan Sun [République populaire de Chine] ; Yanhua Fan [République populaire de Chine] ; Dan Jin [République populaire de Chine] ; Yan Pei [République populaire de Chine]Source :
- Journal of invertebrate pathology [ 1096-0805 ] ; 2020.
Abstract
FK506 binding proteins (FKBPs) participate in regulation of diverse biological processes. However, the role of these proteins in insect-pathogenic fungi is far from well understood. To investigate the functions of FKBPs in Beauveria bassiana, a widely used entomopathogenic fungus for control of insect pests, we identify three putative FKBP genes, Bbfkbp12, Bbfkbp15, and Bbfkbp50, in the fungus. Gene-disruption experiments show that loss of Bbfkbp12 results in a significant increase of resistance of B. bassiana against the immunosuppressive compounds FK506 and rapamycin, while loss of Bbfkbp50 leads to the resistance to the ergosterol synthesis inhibitor lovastatin. Transcription assays of calcineurin (CaN)- and mTOR (mammalian target of rapamycin)-downstream target genes confirm that BbFKBP12 is the target of both FK506 and rapamycin, associated with CaN- and mTOR-signal pathways in B. bassiana. GFP-tagging of the proteins shows that BbFKBP12 and BbFKBP15 localize in cytoplasm while BbFKBP50 in nucleus. Our results provide useful information for the study of functions of CaN- and mTOR-mediated signaling, and ergosterol synthesis in the entomopathogenic fungi.
DOI: 10.1016/j.jip.2020.107334
PubMed: 32006551
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.</title><author><name sortKey="Li, Yujie" sort="Li, Yujie" uniqKey="Li Y" first="Yujie" last="Li">Yujie Li</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Hui" sort="Ren, Hui" uniqKey="Ren H" first="Hui" last="Ren">Hui Ren</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Yutao" sort="Zhao, Yutao" uniqKey="Zhao Y" first="Yutao" last="Zhao">Yutao Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Jiyuan" sort="Sun, Jiyuan" uniqKey="Sun J" first="Jiyuan" last="Sun">Jiyuan Sun</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Fan, Yanhua" sort="Fan, Yanhua" uniqKey="Fan Y" first="Yanhua" last="Fan">Yanhua Fan</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Dan" sort="Jin, Dan" uniqKey="Jin D" first="Dan" last="Jin">Dan Jin</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Pei, Yan" sort="Pei, Yan" uniqKey="Pei Y" first="Yan" last="Pei">Yan Pei</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China. Electronic address: peiyan3@swu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32006551</idno><idno type="pmid">32006551</idno><idno type="doi">10.1016/j.jip.2020.107334</idno><idno type="wicri:Area/Main/Corpus">000129</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000129</idno><idno type="wicri:Area/Main/Curation">000129</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000129</idno><idno type="wicri:Area/Main/Exploration">000129</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.</title><author><name sortKey="Li, Yujie" sort="Li, Yujie" uniqKey="Li Y" first="Yujie" last="Li">Yujie Li</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Hui" sort="Ren, Hui" uniqKey="Ren H" first="Hui" last="Ren">Hui Ren</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Yutao" sort="Zhao, Yutao" uniqKey="Zhao Y" first="Yutao" last="Zhao">Yutao Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Jiyuan" sort="Sun, Jiyuan" uniqKey="Sun J" first="Jiyuan" last="Sun">Jiyuan Sun</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Fan, Yanhua" sort="Fan, Yanhua" uniqKey="Fan Y" first="Yanhua" last="Fan">Yanhua Fan</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Dan" sort="Jin, Dan" uniqKey="Jin D" first="Dan" last="Jin">Dan Jin</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author><author><name sortKey="Pei, Yan" sort="Pei, Yan" uniqKey="Pei Y" first="Yan" last="Pei">Yan Pei</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China. Electronic address: peiyan3@swu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716</wicri:regionArea><wicri:noRegion>Chongqing 400716</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of invertebrate pathology</title><idno type="eISSN">1096-0805</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">FK506 binding proteins (FKBPs) participate in regulation of diverse biological processes. However, the role of these proteins in insect-pathogenic fungi is far from well understood. To investigate the functions of FKBPs in Beauveria bassiana, a widely used entomopathogenic fungus for control of insect pests, we identify three putative FKBP genes, Bbfkbp12, Bbfkbp15, and Bbfkbp50, in the fungus. Gene-disruption experiments show that loss of Bbfkbp12 results in a significant increase of resistance of B. bassiana against the immunosuppressive compounds FK506 and rapamycin, while loss of Bbfkbp50 leads to the resistance to the ergosterol synthesis inhibitor lovastatin. Transcription assays of calcineurin (CaN)- and mTOR (mammalian target of rapamycin)-downstream target genes confirm that BbFKBP12 is the target of both FK506 and rapamycin, associated with CaN- and mTOR-signal pathways in B. bassiana. GFP-tagging of the proteins shows that BbFKBP12 and BbFKBP15 localize in cytoplasm while BbFKBP50 in nucleus. Our results provide useful information for the study of functions of CaN- and mTOR-mediated signaling, and ergosterol synthesis in the entomopathogenic fungi.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32006551</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0805</ISSN><JournalIssue CitedMedium="Internet"><Volume>171</Volume><PubDate><Year>2020</Year><Month>03</Month></PubDate></JournalIssue><Title>Journal of invertebrate pathology</Title><ISOAbbreviation>J Invertebr Pathol</ISOAbbreviation></Journal><ArticleTitle>Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.</ArticleTitle><Pagination><MedlinePgn>107334</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0022-2011(19)30422-7</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jip.2020.107334</ELocationID><Abstract><AbstractText>FK506 binding proteins (FKBPs) participate in regulation of diverse biological processes. However, the role of these proteins in insect-pathogenic fungi is far from well understood. To investigate the functions of FKBPs in Beauveria bassiana, a widely used entomopathogenic fungus for control of insect pests, we identify three putative FKBP genes, Bbfkbp12, Bbfkbp15, and Bbfkbp50, in the fungus. Gene-disruption experiments show that loss of Bbfkbp12 results in a significant increase of resistance of B. bassiana against the immunosuppressive compounds FK506 and rapamycin, while loss of Bbfkbp50 leads to the resistance to the ergosterol synthesis inhibitor lovastatin. Transcription assays of calcineurin (CaN)- and mTOR (mammalian target of rapamycin)-downstream target genes confirm that BbFKBP12 is the target of both FK506 and rapamycin, associated with CaN- and mTOR-signal pathways in B. bassiana. GFP-tagging of the proteins shows that BbFKBP12 and BbFKBP15 localize in cytoplasm while BbFKBP50 in nucleus. Our results provide useful information for the study of functions of CaN- and mTOR-mediated signaling, and ergosterol synthesis in the entomopathogenic fungi.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yujie</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Yutao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Jiyuan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Yanhua</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Dan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pei</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, China. Electronic address: peiyan3@swu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Invertebr Pathol</MedlineTA><NlmUniqueID>0014067</NlmUniqueID><ISSNLinking>0022-2011</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Beauveria bassiana</Keyword><Keyword MajorTopicYN="Y">FK506</Keyword><Keyword MajorTopicYN="Y">FK506-binding protein</Keyword><Keyword MajorTopicYN="Y">Lovastatin</Keyword><Keyword MajorTopicYN="Y">Rapamycin</Keyword><Keyword MajorTopicYN="Y">Resistance</Keyword></KeywordList><CoiStatement>Declaration of Competing Interests The authors declare that they have no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>01</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32006551</ArticleId><ArticleId IdType="pii">S0022-2011(19)30422-7</ArticleId><ArticleId IdType="doi">10.1016/j.jip.2020.107334</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Yujie" sort="Li, Yujie" uniqKey="Li Y" first="Yujie" last="Li">Yujie Li</name></noRegion><name sortKey="Fan, Yanhua" sort="Fan, Yanhua" uniqKey="Fan Y" first="Yanhua" last="Fan">Yanhua Fan</name><name sortKey="Jin, Dan" sort="Jin, Dan" uniqKey="Jin D" first="Dan" last="Jin">Dan Jin</name><name sortKey="Pei, Yan" sort="Pei, Yan" uniqKey="Pei Y" first="Yan" last="Pei">Yan Pei</name><name sortKey="Ren, Hui" sort="Ren, Hui" uniqKey="Ren H" first="Hui" last="Ren">Hui Ren</name><name sortKey="Sun, Jiyuan" sort="Sun, Jiyuan" uniqKey="Sun J" first="Jiyuan" last="Sun">Jiyuan Sun</name><name sortKey="Zhao, Yutao" sort="Zhao, Yutao" uniqKey="Zhao Y" first="Yutao" last="Zhao">Yutao Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000136 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000136 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32006551
|texte= Characterization of three FK506-binding proteins in the entomopathogenic fungus Beauveria bassiana.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32006551" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |